Cell stiffness, contractile stress and the role of extracellular matrix

Steven S An; Jina Kim; Kwangmi Ahn; Xavier Trepat; Kenneth J Drake; Sarvesh Kumar; Guoyu Ling; Carolyn Purington; Tirumalai Rangasamy; Thomas W Kensler; Wayne Mitzner; Jeffrey J Fredberg; Shyam Biswal

doi:10.1016/j.bbrc.2009.03.118

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Cell stiffness, contractile stress and the role of extracellular matrix

Journal article

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Cell stiffness, contractile stress and the role of extracellular matrix

Steven S An, Jina Kim, Kwangmi Ahn, Xavier Trepat, Kenneth J Drake, Sarvesh Kumar, Guoyu Ling, Carolyn Purington, Tirumalai Rangasamy, Thomas W Kensler, …

Biochemical and biophysical research communications, Vol.382(4), pp.697-703

2009

DOI: https://doi.org/10.1016/j.bbrc.2009.03.118

PMCID: PMC2956180

PMID: 19327344

Abstract

Oxidative stress

Airway hyperreactivity

Nrf2

Airway smooth muscle

ECM

Asthma

Here we have assessed the effects of extracellular matrix (ECM) composition and rigidity on mechanical properties of the human airway smooth muscle (ASM) cell. Cell stiffness and contractile stress showed appreciable changes from the most relaxed state to the most contracted state: we refer to the maximal range of these changes as the cell contractile scope. The contractile scope was least when the cell was adherent upon collagen V, followed by collagen IV, laminin, and collagen I, and greatest for fibronectin. Regardless of ECM composition, upon adherence to increasingly rigid substrates, the ASM cell positively regulated expression of antioxidant genes in the glutathione pathway and heme oxygenase, and disruption of a redox-sensitive transcription factor, nuclear erythroid 2 p45-related factor (Nrf2), culminated in greater contractile scope. These findings provide biophysical evidence that ECM differentially modulates muscle contractility and, for the first time, demonstrate a link between muscle contractility and Nrf2-directed responses.

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Title: Cell stiffness, contractile stress and the role of extracellular matrix
Creators: Steven S An - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Jina Kim - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Kwangmi Ahn - Division of Biostatistics, Penn State College of Medicine, Hershey, PA 17033, USA
Xavier Trepat - CIBER, Enfermedades Respiratorias, 07110 Bunyola, Spain
Kenneth J Drake - Division of Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, MA 02115, USA
Sarvesh Kumar - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Guoyu Ling - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Carolyn Purington - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Tirumalai Rangasamy - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Thomas W Kensler - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Wayne Mitzner - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Jeffrey J Fredberg - Division of Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, MA 02115, USA
Shyam Biswal - Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Room E-7616, Baltimore, MD 21205, USA
Publication Details: Biochemical and biophysical research communications, Vol.382(4), pp.697-703
Date published: 2009
Publisher: Elsevier Inc
Academic Unit: Clinical and Translational Research
Language: English
Resource Type: Journal article
Identifiers: 991031654596704646