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Use of a brine shrimp assay to study herbal teas in the classroom
Teaching activity   Open access   Peer reviewed

Use of a brine shrimp assay to study herbal teas in the classroom

Annette Opler, Rebecca Mizell, Alexander Robert, Miguel Cervantes-Cervantes, Dwight Kincaid and Edward J. Kennelly
The American biology teacher, Vol.64(8), pp.596-604
10/01/2002

Abstract

Education Phytochemistry
The use of herbal products as medicinal remedies has increased greatly in the past decade. We are inundated with advertisements that tell us natural remedies will accomplish cures and imply that the term "natural" means free of side effects. Unfortunately, only a few of the herbal remedies have scientific data to back up their clinical bene­fits and some are known to be dangerous. Herbal teas remain an underinvestigated group of plant products. The brine shrimp assay is an excellent tool with which to begin such investigations of an herb's biological activity. J.L. McLaughlin and his associates (Meyer et al., 1982; McLaughlin & Rogers, 1998) developed this assay and it has been used to identify potent anti-cancer compounds. This in vivo assay is a reliable prescreen for the bio­logical activity of plant secondary metabolites and it has been used successfully in the classroom (Kendler et al., 1992).
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