A Conserved HEAT Domain within eIF4G Directs Assembly of the Translation Initiation Machinery

Joseph Marcotrigiano; Ivan B Lomakin; Nahum Sonenberg; Tatyana V Pestova; Christopher U.T Hellen; Stephen K Burley

doi:10.1016/S1097-2765(01)00167-8

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A Conserved HEAT Domain within eIF4G Directs Assembly of the Translation Initiation Machinery

Journal article

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A Conserved HEAT Domain within eIF4G Directs Assembly of the Translation Initiation Machinery

Joseph Marcotrigiano, Ivan B Lomakin, Nahum Sonenberg, Tatyana V Pestova, Christopher U.T Hellen and Stephen K Burley

Molecular cell, Vol.7(1), pp.193-203

2001

DOI: https://doi.org/10.1016/S1097-2765(01)00167-8

PMID: 11172724

Abstract

The X-ray structure of the phylogenetically conserved middle portion of human e ukaryotic i nitiation f actor (eIF) 4GII has been determined at 2.4 Å resolution, revealing a crescent-shaped domain consisting of ten α helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA i nternal r ibosome e ntry s ite (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation.

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Title: A Conserved HEAT Domain within eIF4G Directs Assembly of the Translation Initiation Machinery
Creators: Joseph Marcotrigiano - Laboratories of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA
Ivan B Lomakin - Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science, Center at Brooklyn, Brooklyn, New York 11203-2098, USA
Nahum Sonenberg - Department of Biochemistry and, McGill Cancer Center, McGill University, Montreal, Quebec H3G 1Y6, Canada
Tatyana V Pestova - Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science, Center at Brooklyn, Brooklyn, New York 11203-2098, USA
Christopher U.T Hellen - Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science, Center at Brooklyn, Brooklyn, New York 11203-2098, USA
Stephen K Burley - Laboratories of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA
Publication Details: Molecular cell, Vol.7(1), pp.193-203
Date published: 2001
Publisher: Elsevier Inc
Academic Unit: Chemistry and Chemical Biology (SAS); Institute for Quantitative Biomedicine
Language: English
Resource Type: Journal article
Identifiers: 991031665185004646