A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter

Journal article

Open access

Peer reviewed

Ferez S Nallaseth and Stephen Anderson

Microbial cell factories, Vol.12(1), pp.36-36

04/19/2013

PMCID: PMC3654994

PMID: 23602005

Abstract

Technical Notes

Chemical mutagenesis

Cellular phosphatase reference activity

AOX1

α-sec-EAP (α-secMAT

P. pastoris

Protein export

Dual component chromogenic and immuno-chromogenic stains

Over-secretor mutants

E.coli Alkaline Phosphatase

url

https://doi.org/10.1186/1475-2859-12-36View

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Title: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter
Creators: Ferez S Nallaseth - Department for Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine Rutgers, The State University of New Jersey, 679 Hoes Lane West, Piscataway, NJ 08854, USA
Stephen Anderson - Department for Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine Rutgers, The State University of New Jersey, 679 Hoes Lane West, Piscataway, NJ 08854, USA
Publication Details: Microbial cell factories, Vol.12(1), pp.36-36
Date published: 04/19/2013
Publisher: BioMed Central
Academic Unit: Molecular Biology and Biochemistry (SAS)
Language: English
Resource Type: Journal article
Identifiers: 991031653875704646