An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue

Tracy Goodpaster; Aster Legesse-Miller; Meera R Hameed; Seena C Aisner; Julie Randolph-Habecker; Hilary A Coller

doi:10.1369/jhc.7A7287.2007

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An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue

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An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue

Tracy Goodpaster, Aster Legesse-Miller, Meera R Hameed, Seena C Aisner, Julie Randolph-Habecker and Hilary A Coller

The journal of histochemistry and cytochemistry, Vol.56(4), pp.347-358

04/2008

DOI: https://doi.org/10.1369/jhc.7A7287.2007

PMCID: PMC2326106

PMID: 18071065

Abstract

TE-7

fibroblasts

immunohistochemistry

Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors. (J Histochem Cytochem 56:347–358, 2008)

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Title: An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue
Creators: Tracy Goodpaster - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Aster Legesse-Miller - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Meera R Hameed - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Seena C Aisner - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Julie Randolph-Habecker - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Hilary A Coller - Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)
Publication Details: The journal of histochemistry and cytochemistry, Vol.56(4), pp.347-358
Date published: 04/2008
Publisher: Histochemical Society
Academic Unit: Pathology, Immunology and Laboratory Medicine (NJMS)
Language: English
Resource Type: Journal article
Identifiers: 991031654662804646