Apoptosis Is Associated with Modifications of bcl-2 mRNA AU-Binding Proteins

Martino Donnini; Andrea Lapucci; Laura Papucci; Ewa Witort; Alessio Tempestini; Gary Brewer; Anna Bevilacqua; Angelo Nicolin; Sergio Capaccioli; Nicola Schiavone

doi:10.1006/bbrc.2001.5700

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Apoptosis Is Associated with Modifications of bcl-2 mRNA AU-Binding Proteins

Journal article

Peer reviewed

Apoptosis Is Associated with Modifications of bcl-2 mRNA AU-Binding Proteins

Martino Donnini, Andrea Lapucci, Laura Papucci, Ewa Witort, Alessio Tempestini, Gary Brewer, Anna Bevilacqua, Angelo Nicolin, Sergio Capaccioli and Nicola Schiavone

Biochemical and biophysical research communications, Vol.287(5), pp.1063-1069

10/12/2001

DOI: https://doi.org/10.1006/bbrc.2001.5700

PMID: 11587529

Abstract

apoptosis

AU-binding proteins

AU-rich elements

bcl-2

gene expression

posttranscriptional control

The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3′-untranslated region (3′-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.

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Title: Apoptosis Is Associated with Modifications of bcl-2 mRNA AU-Binding Proteins
Creators: Martino Donnini - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Andrea Lapucci - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Laura Papucci - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Ewa Witort - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Alessio Tempestini - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Gary Brewer - Department of Molecular Genetics and Microbiology, UMDNJ, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey, 08854
Anna Bevilacqua - Department of Molecular Genetics and Microbiology, UMDNJ, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey, 08854
Angelo Nicolin - Department of Pharmacology, School of Medicine, University of Milan, Italy
Sergio Capaccioli - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Nicola Schiavone - Department of Experimental Pathology and Oncology, School of Medicine, University of Florence, Italy
Publication Details: Biochemical and biophysical research communications, Vol.287(5), pp.1063-1069
Date published: 10/12/2001
Publisher: Elsevier Inc
Academic Unit: Biochemistry and Molecular Biology (RWJMS)
Language: English
Resource Type: Journal article
Identifiers: 991031665961504646