Abstract
We measured the intensity ratio of wide-to-narrow angle scatter, optical scatter image ratio (OSIR), in single cells during apoptosis and after overexpression of the mitochondria-bound antiapoptotic protein BCL-x
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. OSIR is sensitive to particle size/shape for objects with wavelength-scale dimensions, and was used as a morphometric measure of cellular response. Three cell variants were treated with staurosporine (STS): nontransfected parental CSM14.1, CSM14.1 stably expressing yellow fluorescent protein (YFP) with diffuse YFP fluorescence, and apoptosis-resistant CSM14.1 stably expressing the fusion protein construct YFP-BCL-x
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with YFP fluorescence localized on the mitochondria. After treatment with 1 or 2
μ
M STS, the measured OSIR decreased monotonically by ∼25% in the nontransfected and YFP variants, and reached a steady-state value 40–60 min after STS treatment. The decrease in OSIR at the onset of apoptosis preceded phosphatidyl serine exposure by 5 h. In the YFP-BCL-x
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cell variant, the initial OSIR was already ∼24% lower than the initial OSIR in YFP and nontransfected cells, and only decreased by <10% after STS treatment. Alterations in light scattering by cells overexpressing BCL-x
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even before apoptosis induction raise interesting questions as to the role of BCL-x
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in conferring apoptosis resistance by preconditioning the cells and possibly altering mitochondrial morphology.