Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Collette Fitzgerald; Zheng Chao Tu; Mary Patrick; Tracy Stiles; Andy J Lawson; Monica Santovenia; Maarten J Gilbert; Marcel van Bergen; Kevin Joyce; Janet Pruckler; Steven Stroika; Birgitta Duim; William G Miller; Vladimir Loparev; Jan C Sinnige; Patricia I Fields; Robert V Tauxe; Martin J Blaser; Jaap A Wagenaar

doi:10.1099/ijs.0.057778-0

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Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Journal article

Open access

Peer reviewed

Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Collette Fitzgerald, Zheng Chao Tu, Mary Patrick, Tracy Stiles, Andy J Lawson, Monica Santovenia, Maarten J Gilbert, Marcel van Bergen, Kevin Joyce, Janet Pruckler, …

International journal of systematic and evolutionary microbiology, Vol.64(Pt 9), pp.2944-2948

09/2014

DOI: https://doi.org/10.1099/ijs.0.057778-0

PMID: 24899653

Abstract

Amplified Fragment Length Polymorphism Analysis

Animals

Bacterial Typing Techniques

Campylobacter fetus - classification

Campylobacter fetus - genetics

Campylobacter fetus - isolation & purification

DNA, Bacterial - genetics

Humans

Molecular Sequence Data

Nucleic Acid Hybridization

Phylogeny

Reptiles - microbiology

RNA, Ribosomal, 16S - genetics

Sequence Analysis, DNA

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

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Title: Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles
Creators: Collette Fitzgerald - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Zheng Chao Tu - Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
Mary Patrick - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Tracy Stiles - Massachusetts Department of Public Health, Jamaica Plain, MA, USA
Andy J Lawson - Public Health England, London, UK
Monica Santovenia - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Maarten J Gilbert - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Marcel van Bergen - WHO Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands
Kevin Joyce - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Janet Pruckler - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Steven Stroika - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Birgitta Duim - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
William G Miller - USDA, ARS, WRRC, Produce Safety and Microbiology Research Unit, Albany, CA, USA
Vladimir Loparev - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Jan C Sinnige - Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
Patricia I Fields - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Robert V Tauxe - Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA
Martin J Blaser - Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
Jaap A Wagenaar - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Publication Details: International journal of systematic and evolutionary microbiology, Vol.64(Pt 9), pp.2944-2948
Date published: 09/2014
Academic Unit: Center for Advanced Biotechnology and Medicine
Language: English
Resource Type: Journal article
Identifiers: 991031665528504646