Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs

Xiangyue Wu; Sandra Chesoni; Gaelle Rondeau; Christi Tempesta; Reshma Patel; Sandy Charles; Naznin Daginawala; Beth E Zucconi; Aparna Kishor; Guangwu Xu; Yufang Shi; Mei-Ling Li; Patricia Irizarry-Barreto; John Welsh; Gerald M Wilson; Gary Brewer

doi:10.1093/nar/gks1453

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Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs

Journal article

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Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs

Xiangyue Wu, Sandra Chesoni, Gaelle Rondeau, Christi Tempesta, Reshma Patel, Sandy Charles, Naznin Daginawala, Beth E Zucconi, Aparna Kishor, Guangwu Xu, …

Nucleic acids research, Vol.41(4), pp.2644-2658

02/2013

DOI: https://doi.org/10.1093/nar/gks1453

PMCID: PMC3575833

PMID: 23303783

Abstract

RNA

The RNA-binding protein AUF1 binds AU-rich elements in 3′-untranslated regions to regulate mRNA degradation and/or translation. Many of these mRNAs are predicted microRNA targets as well. An emerging theme in post-transcriptional control of gene expression is that RNA-binding proteins and microRNAs co-regulate mRNAs. Recent experiments and bioinformatic analyses suggest this type of co-regulation may be widespread across the transcriptome. Here, we identified mRNA targets of AUF1 from a complex pool of cellular mRNAs and examined a subset of these mRNAs to explore the links between RNA binding and mRNA degradation for both AUF1 and Argonaute 2 (AGO2), which is an essential effector of microRNA-induced gene silencing. Depending on the specific mRNA examined, AUF1 and AGO2 binding is proportional/cooperative, reciprocal/competitive or independent. For most mRNAs in which AUF1 affects their decay rates, mRNA degradation requires AGO2. Thus, AUF1 and AGO2 present mRNA-specific allosteric binding relationships for co-regulation of mRNA degradation.

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Title: Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs
Creators: Xiangyue Wu - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Sandra Chesoni - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Gaelle Rondeau - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Christi Tempesta - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Reshma Patel - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Sandy Charles - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Naznin Daginawala - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Beth E Zucconi - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Aparna Kishor - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Guangwu Xu - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Yufang Shi - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Mei-Ling Li - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Patricia Irizarry-Barreto - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
John Welsh - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Gerald M Wilson - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Gary Brewer - Department of Biochemistry and Molecular Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Publication Details: Nucleic acids research, Vol.41(4), pp.2644-2658
Date published: 02/2013
Publisher: Oxford University Press
Academic Unit: Instructional Support (SAS); Biochemistry and Molecular Biology (RWJMS)
Language: English
Resource Type: Journal article
Identifiers: 991031665786704646