Crystallization and preliminary X-ray diffraction studies of coxsackievirus B1

Thomas Li; Anqiang Zhang; Narushi Iizuka; Akio Nomoto; Edward Arnold

doi:10.1016/0022-2836(92)90268-O

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$Crystallization and preliminary X-ray diffraction studies of coxsackievirus B1$

Journal article

Peer reviewed

Crystallization and preliminary X-ray diffraction studies of coxsackievirus B1

Thomas Li, Anqiang Zhang, Narushi Iizuka, Akio Nomoto and Edward Arnold

Journal of molecular biology, Vol.223(4), pp.1171-1175

1992

DOI: https://doi.org/10.1016/0022-2836(92)90268-O

PMID: 1311388

Abstract

crystallization

X-ray diffraction

virus structure

synchrotron radiation

coxsackieviruses

Preparations of coxsackievirus B1 (CVB1) derived from an infectious cDNA clone have been crystallized in multiple crystal forms. Using high intensity synchrotron radiation, an orthorhombic form of the crystals was shown to diffract X-rays to at least 2.9 Å resolution. The unit cell has a primitive lattice with dimensions a = 323 A ̊ , b = 450 A ̊ , and c = 522 A ̊ . A crystallographic asymmetric unit of these CVB1 crystals probably contains an entire virus particle, implying the presence of 60-fold non-crystallographic redundancy. This CVB1 crystal form appears to be suitable for high-resolution structure determination by X-ray crystallography.

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Details

Title: Crystallization and preliminary X-ray diffraction studies of coxsackievirus B1
Creators: Thomas Li - Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry, 679 Hoes Lane, Piscataway, NJ 08854-5638, U.S.A
Anqiang Zhang - Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry, 679 Hoes Lane, Piscataway, NJ 08854-5638, U.S.A
Narushi Iizuka - Department of Microbiology, The Tokyo Metropolitan Institute of Medical Research, Tokyo, Japan
Akio Nomoto - Department of Microbiology, The Tokyo Metropolitan Institute of Medical Research, Tokyo, Japan
Edward Arnold - Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry, 679 Hoes Lane, Piscataway, NJ 08854-5638, U.S.A
Publication Details: Journal of molecular biology, Vol.223(4), pp.1171-1175
Date published: 1992
Publisher: Elsevier Ltd
Academic Unit: Center for Advanced Biotechnology and Medicine
Language: English
Resource Type: Journal article
Identifiers: 991031654655304646