DNA-mediated gene transfer into epidermal cells using electroporation

M Reiss; M.M Jastreboff; J.R Bertino; R Narayanan

doi:10.1016/0006-291X(86)91202-7

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DNA-mediated gene transfer into epidermal cells using electroporation

Journal article

Peer reviewed

DNA-mediated gene transfer into epidermal cells using electroporation

M Reiss, M.M Jastreboff, J.R Bertino and R Narayanan

Biochemical and biophysical research communications, Vol.137(1), pp.244-249

1986

DOI: https://doi.org/10.1016/0006-291X(86)91202-7

PMID: 3459459

Abstract

A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10 −6 cells per μg of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.

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Title: DNA-mediated gene transfer into epidermal cells using electroporation
Creators: M Reiss - Department of Medicine, Yale University School of Medicine, New Haven, CT 06510 USA
M.M Jastreboff - Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510 USA
J.R Bertino - Department of Medicine, Yale University School of Medicine, New Haven, CT 06510 USA
R Narayanan - Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510 USA
Publication Details: Biochemical and biophysical research communications, Vol.137(1), pp.244-249
Date published: 1986
Publisher: Elsevier Inc
Academic Unit: Bertino Office
Language: English
Resource Type: Journal article
Identifiers: 991031665817904646