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Detection of Listeria monocytogenes by using the polymerase chain reaction
Journal article   Open access  Peer reviewed

Detection of Listeria monocytogenes by using the polymerase chain reaction

M T Bessesen, Q A Luo, H A Rotbart, M J Blaser and R T Ellison
Applied and environmental microbiology, Vol.56(9), pp.2930-2932
09/1990
PMCID: PMC184869
PMID: 2125817

Abstract

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
url
https://doi.org/10.1128/aem.56.9.2930-2932.1990View
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