Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound

Senjie Lin; Huan Zhang; Yubo Hou; Lilibeth Miranda; Debashish Bhattacharya

doi:10.1128/AEM.00586-06

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Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound

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Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound

Senjie Lin, Huan Zhang, Yubo Hou, Lilibeth Miranda and Debashish Bhattacharya

Applied and environmental microbiology, Vol.72(8), pp.5626-5630

08/2006

DOI: https://doi.org/10.1128/AEM.00586-06

PMCID: PMC1538694

PMID: 16885319

Abstract

Microbial Ecology

We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya -like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems.

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Title: Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound
Creators: Senjie Lin - Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Huan Zhang - Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Yubo Hou - Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Lilibeth Miranda - Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Debashish Bhattacharya - Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340
Publication Details: Applied and environmental microbiology, Vol.72(8), pp.5626-5630
Date published: 08/2006
Publisher: American Society for Microbiology
Academic Unit: Biochemistry and Microbiology (SEBS)
Language: English
Resource Type: Journal article
Identifiers: 991031665010904646