IV. Molecular biology of S-layers

Hubert Bahl; Holger Scholz; Nicolas Bayan; Mohamed Chami; Gérard Leblon; Thaddée Gulik-Krzywicki; Emanuel Shechter; Agnés Fouet; Stéphane Mesnage; Evelyne Tosi-Couture; Pierre Gounon; Michèle Mock; Everly Conway de Macario; Alberto J.L Macario; Luis A Fernández-Herrero; Garbiñe Olabarrı́a; José Berenguer; Martin J Blaser; Beatrix Kuen; Werner Lubitz; Margit Sára; Peter H Pouwels; Carin P.A.M Kolen; Hein J Boot; Airi Palva; Michaela Truppe; Stephan Howorka; Gerhard Schroll; Sonja Lechleitner; Stephanie Resch

doi:10.1016/S0168-6445(97)00050-8

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IV. Molecular biology of S-layers

Hubert Bahl, Holger Scholz, Nicolas Bayan, Mohamed Chami, Gérard Leblon, Thaddée Gulik-Krzywicki, Emanuel Shechter, Agnés Fouet, Stéphane Mesnage, Evelyne Tosi-Couture, …

FEMS microbiology reviews, Vol.20(1), pp.47-98

1997

DOI: https://doi.org/10.1016/S0168-6445(97)00050-8

Abstract

ABC transporter

Antigenic diversity

Bacillus anthracis

Bacillus stearothermophilus

Corynebacterium glutamicum

cspB gene

EA1 protein

Gene regulation

Heterologous expression

Lactobacilli

Lactobacillus brevis

Methanogenic archaea

Methanosarcina mazei

Oxygen stress

PS2 protein

S-layer

S-layer gene

S-layer protein

S-layer structure-function relationship

S-layer variation

Sap protein

sbs gene

SbsA/SbsB fusion protein

Secretion of β-lactamase

SLH motif

slp gene

slpA gene

Thermus thermophilus

In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins form the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7–8 S-layer proteins with a high degree of homology at the 5′ and 3′ ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finally, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology.

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Title: IV. Molecular biology of S-layers
Creators: Hubert Bahl - Fachbereich Biologie, Mikrobiologie, Universität Rostock, D-18051 Rostock, Germany
Holger Scholz - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Nicolas Bayan - Laboratoire des Biomembranes, URA 1116 CNRS, Université de Paris-Sud, F-91405 Orsay, France
Mohamed Chami - Laboratoire des Biomembranes, URA 1116 CNRS, Université de Paris-Sud, F-91405 Orsay, France
Gérard Leblon - Laboratoire de Biologie Moléculaire des Corynebactéries, URA 1354 CNRS, Université de Paris-Sud, F-91405 Orsay, France
Thaddée Gulik-Krzywicki - Centre de Génétique Moléculaire, CNRS, F-91190 Gif sur Yvette, France
Emanuel Shechter - Laboratoire des Biomembranes, URA 1116 CNRS, Université de Paris-Sud, F-91405 Orsay, France
Agnés Fouet - Laboratoire de Génétique Moléculaire des Toxines (CNRS URA 1858 CNRS), Institut Pasteur, 28, rue du Dr Roux, F-75724 Paris Cedex 15, France
Stéphane Mesnage - Laboratoire de Génétique Moléculaire des Toxines (CNRS URA 1858 CNRS), Institut Pasteur, 28, rue du Dr Roux, F-75724 Paris Cedex 15, France
Evelyne Tosi-Couture - Station Centrale de Microscopie Electronique, Institut Pasteur, 28, rue du Dr. Roux, F-75724 Paris Cedex 15, France
Pierre Gounon - Station Centrale de Microscopie Electronique, Institut Pasteur, 28, rue du Dr. Roux, F-75724 Paris Cedex 15, France
Michèle Mock - Laboratoire de Génétique Moléculaire des Toxines (CNRS URA 1858 CNRS), Institut Pasteur, 28, rue du Dr Roux, F-75724 Paris Cedex 15, France
Everly Conway de Macario - Wadsworth Center, Division of Molecular Medicine, New York State Department of Health and Department of Biomedical Sciences, School of Public Health, The University at Albany, Albany, NY 12201-0509, USA
Alberto J.L Macario - Wadsworth Center, Division of Molecular Medicine, New York State Department of Health and Department of Biomedical Sciences, School of Public Health, The University at Albany, Albany, NY 12201-0509, USA
Luis A Fernández-Herrero - Centro de Biologı́a Molecular `Severo Ochoa', Universidad Autónoma de Madrid, E-28049 Madrid, Spain
Garbiñe Olabarrı́a - Centro de Biologı́a Molecular `Severo Ochoa', Universidad Autónoma de Madrid, E-28049 Madrid, Spain
José Berenguer - Centro de Biologı́a Molecular `Severo Ochoa', Universidad Autónoma de Madrid, E-28049 Madrid, Spain
Martin J Blaser - Division of Infectious Diseases, Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Beatrix Kuen - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Werner Lubitz - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Margit Sára - Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria
Peter H Pouwels - TNO Nutrition and Food Research Institute, P.O. Box 5815, NL-2280 HV Rijswijk, The Netherlands
Carin P.A.M Kolen - BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands
Hein J Boot - BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands
Airi Palva - Agricultural Research Centre of Finland, Food Research Institute, FIN-31600 Jokioinen, Finland
Michaela Truppe - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Stephan Howorka - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Gerhard Schroll - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Sonja Lechleitner - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Stephanie Resch - Institut für Mikrobiologie und Genetik, Universität Wien, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria
Publication Details: FEMS microbiology reviews, Vol.20(1), pp.47-98
Date published: 1997
Publisher: Elsevier B.V
Academic Unit: Center for Advanced Biotechnology and Medicine
Language: English
Resource Type: Journal article
Identifiers: 991031666047104646