Sign in
Back
In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation
Journal article   Peer reviewed

In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

R Narayanan, M M Jastreboff, C F Chiu and J R Bertino
Biochemical and biophysical research communications, Vol.141(3), pp.1018-1024
12/30/1986
DOI: https://doi.org/10.1016/S0006-291X(86)80146-2
PMID: 3468946

Abstract

Acetyltransferases - genetics Acetyltransferases - metabolism Animals Bone Marrow Cells Chloramphenicol O-Acetyltransferase Colony-Forming Units Assay Electricity Female Granulocytes - enzymology Hematopoietic Stem Cells - enzymology Macrophages - enzymology Mice Mice, Inbred CBA Plasmids Transfection
Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

Metrics

3 Record Views
28 Times Cited - Web of Science

Details