Microarray analysis of gene expression in eastern oyster ( Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo ( Perkinsus marinus) challenge

Shaolin Wang; Eric Peatman; Hong Liu; David Bushek; Susan E Ford; Huseyin Kucuktas; Jonas Quilang; Ping Li; Richard Wallace; Yongping Wang; Ximing Guo; Zhanjiang Liu

doi:10.1016/j.fsi.2010.07.035

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Microarray analysis of gene expression in eastern oyster ( Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo ( Perkinsus marinus) challenge

Journal article

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Microarray analysis of gene expression in eastern oyster ( Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo ( Perkinsus marinus) challenge

Shaolin Wang, Eric Peatman, Hong Liu, David Bushek, Susan E Ford, Huseyin Kucuktas, Jonas Quilang, Ping Li, Richard Wallace, Yongping Wang, …

Fish & shellfish immunology, Vol.29(6), pp.921-929

2010

DOI: https://doi.org/10.1016/j.fsi.2010.07.035

PMID: 20708691

Abstract

Antimicrobial

Dermo

Microarray

Oxidative stress

Oyster

Dermo disease, caused by Perkinsus marinus, is one of the most severe diseases of eastern oysters, Crassostrea virginica. It causes serious mortalities in both wild and aquacultured oysters. Using existing expressed sequence tag (EST) resources, we developed a 12K in situ oligonucleotide microarray and used it for the analysis of gene expression profiles of oysters during the interactions between P. marinus and its oyster host. Significant gene expression regulation was found at day 30 post-challenge in the eastern oyster. Putative identities of the differentially expressed genes revealed a set of genes involved in several processes including putative antimicrobial defenses, pathogen recognition and uptake, anti-oxidation and apoptosis. Consistent with results obtained from previous, smaller-scale experiments, expression profiles revealed a large set of genes likely involved in an active mitigating response to oxidative stress and apoptosis induced by P. marinus. Additionally, a unique galectin from C. virginica, CvGal, which serves as a preferential receptor for P. marinus trophozoites, was found to be significantly down-regulated in gill tissue of oysters with both light and heavy infection, suggesting an attempt to control parasite uptake and proliferation in the later stages of infection. Potential histone-derived antimicrobial responses to P. marinus were also revealed in the gene expression profiles.

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Title: Microarray analysis of gene expression in eastern oyster ( Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo ( Perkinsus marinus) challenge
Creators: Shaolin Wang - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Eric Peatman - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Hong Liu - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
David Bushek - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Ave., Port Norris, NJ 08349, USA
Susan E Ford - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Ave., Port Norris, NJ 08349, USA
Huseyin Kucuktas - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Jonas Quilang - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Ping Li - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Richard Wallace - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Yongping Wang - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Ave., Port Norris, NJ 08349, USA
Ximing Guo - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Ave., Port Norris, NJ 08349, USA
Zhanjiang Liu - The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, 203 Swingle Hall, Auburn University, Auburn, AL 36849, USA
Publication Details: Fish & shellfish immunology, Vol.29(6), pp.921-929
Date published: 2010
Publisher: Elsevier Ltd
Academic Unit: Marine and Coastal Sciences (SEBS)
Language: English
Resource Type: Journal article
Identifiers: 991031664331304646