Primary structure and functional expression of a mammalian skeletal muscle sodium channel

James S Trimmer; Sharon S Cooperman; Sally A Tomiko; Jiuying Zhou; Shelia M Crean; Mary B Boyle; Roland G Kalen; Zuhang Sheng; Robert L Barchi; Frederick J Sigworth; Richard H Goodman; William S Agnew; Gail Mandel

doi:10.1016/0896-6273(89)90113-X

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Primary structure and functional expression of a mammalian skeletal muscle sodium channel

Journal article

Peer reviewed

Primary structure and functional expression of a mammalian skeletal muscle sodium channel

James S Trimmer, Sharon S Cooperman, Sally A Tomiko, Jiuying Zhou, Shelia M Crean, Mary B Boyle, Roland G Kalen, Zuhang Sheng, Robert L Barchi, Frederick J Sigworth, …

Neuron (Cambridge, Mass.), Vol.3(1), pp.33-49

1989

DOI: https://doi.org/10.1016/0896-6273(89)90113-X

PMID: 2559760

Abstract

We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.

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Title: Primary structure and functional expression of a mammalian skeletal muscle sodium channel
Creators: James S Trimmer - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Sharon S Cooperman - Division of Molecular Medicine New England Medical Center Tufts University School of Medicine Boston, Massachusetts 02111, USA
Sally A Tomiko - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Jiuying Zhou - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Shelia M Crean - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Mary B Boyle - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Roland G Kalen - David Mahoney Institute of Neurological Sciences University of Pennsylvania School of Medicine Philadelphia, Pennsylvania 19104, USA
Zuhang Sheng - Division of Molecular Medicine New England Medical Center Tufts University School of Medicine Boston, Massachusetts 02111, USA
Robert L Barchi - David Mahoney Institute of Neurological Sciences University of Pennsylvania School of Medicine Philadelphia, Pennsylvania 19104, USA
Frederick J Sigworth - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Richard H Goodman - Division of Molecular Medicine New England Medical Center Tufts University School of Medicine Boston, Massachusetts 02111, USA
William S Agnew - Department of Cellular and Molecular Physiology Yale University School of Medicine New Haven, Connecticut 06510, USA
Gail Mandel - Division of Molecular Medicine New England Medical Center Tufts University School of Medicine Boston, Massachusetts 02111, USA
Publication Details: Neuron (Cambridge, Mass.), Vol.3(1), pp.33-49
Date published: 1989
Publisher: Elsevier Inc
Academic Unit: Academic Affairs
Language: English
Resource Type: Journal article
Identifiers: 991031665716204646