Abstract
We have evaluated the ability of a new genotyping method for
Campylobacter jejuni
based on restriction fragment length polymorphisms using random chromosomal gene probes. DNAs from
C. jejuni
strains digested with each of three restriction enzymes,
Hha
I,
Hae
III, and
Hpa
II, were analyzed by Southern hybridization using each of two unrelated cosmid clones, P14 and P15 (respectively containing 30- and 35-kb genomic DNA fragments of
C. jejuni
strain OH4384). The method reported provides a stable and discriminating means for identifying
C. jejuni
strains and should be useful for epidemiological analyses.