SH2 domains recognize specific phosphopeptide sequences

Songyang Zhou; Steven E Shoelson; Manas Chaudhuri; Gerald Gish; Tony Pawson; Wayne G Haser; Fred King; Tom Roberts; Sheldon Ratnofsky; Robert J Lechleider; Benjamin G Neel; Raymond B Birge; J.Eduardo Fajardo; Margaret M Chou; Hidesaburo Hanafusa; Brian Schaffhausen; Lewis C Cantley

doi:10.1016/0092-8674(93)90404-E

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SH2 domains recognize specific phosphopeptide sequences

Journal article

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Peer reviewed

SH2 domains recognize specific phosphopeptide sequences

Songyang Zhou, Steven E Shoelson, Manas Chaudhuri, Gerald Gish, Tony Pawson, Wayne G Haser, Fred King, Tom Roberts, Sheldon Ratnofsky, Robert J Lechleider, …

Cell, Vol.72(5), pp.767-778

1993

DOI: https://doi.org/10.1016/0092-8674(93)90404-E

PMID: 7680959

Abstract

A phosphopeptide library was used to determine the sequence specificity of the peptide-binding sites of SH2 domains. One group of SH2 domains (Src, Fyn, Lck, Fgr, Abl, Crk, and Nck) preferred sequences with the general motif pTyr-hydrophilic-hydrophilic-lle/Pro while another group (SH2 domains of p85, phosphollpase C-γ, and SHPTP2) selected the general motif pTyr-hydrophobic-X-hydrophobic. Individual members of these groups selected unique sequences, except the Src subfamily (Src, F́yn, Lck, and Fgr), which all selected the sequence pTyr-Glu-Glu-Ile. The variability in SH2 domain sequences at likely sites of contact provides a structural basis for the phosphopeptide selectivity of these families. Possible in vivo binding sites of the SH2 domains are discussed.

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Title: SH2 domains recognize specific phosphopeptide sequences
Creators: Songyang Zhou - Cellular and Molecular Physiology Harvard Medical School and Department of Medicine Beth Israel Hospital Boston, Massachusetts 02115 USA
Steven E Shoelson - Research Division Joslin Diabetes Center Department of Medicine Brigham and Women's Hospital and Harvard Medical School Boston, Massachusetts 02115 USA
Manas Chaudhuri - Research Division Joslin Diabetes Center Department of Medicine Brigham and Women's Hospital and Harvard Medical School Boston, Massachusetts 02115 USA
Gerald Gish - Division of Molecular and Developmental Biology Samuel Lunenfeld Research Institute Mount Sinai Hospital Toronto, Ontario M5G 1X5, Canada
Tony Pawson - Division of Molecular and Developmental Biology Samuel Lunenfeld Research Institute Mount Sinai Hospital Toronto, Ontario M5G 1X5, Canada
Wayne G Haser - Division of Cellular and Molecular Biology Dana Farber Cancer Institute Harvard Medical School Boston, Massachusetts 02115 USA
Fred King - Division of Cellular and Molecular Biology Dana Farber Cancer Institute Harvard Medical School Boston, Massachusetts 02115 USA
Tom Roberts - Division of Cellular and Molecular Biology Dana Farber Cancer Institute Harvard Medical School Boston, Massachusetts 02115 USA
Sheldon Ratnofsky - BASF Corporation 195 Albany Street Cambridge, Massachusetts 02139 USA
Robert J Lechleider - Molecular Medicine Unit Beth Israel Hospital Boston, Massachusetts 02215 USA
Benjamin G Neel - Molecular Medicine Unit Beth Israel Hospital Boston, Massachusetts 02215 USA
Raymond B Birge - Rockefeller University New York, New York 10021 USA
J.Eduardo Fajardo - Rockefeller University New York, New York 10021 USA
Margaret M Chou - Rockefeller University New York, New York 10021 USA
Hidesaburo Hanafusa - Rockefeller University New York, New York 10021 USA
Brian Schaffhausen - Biochemistry Department Tufts University School of Medicine Boston, Massachusetts 02111 USA
Lewis C Cantley - Cellular and Molecular Physiology Harvard Medical School and Department of Medicine Beth Israel Hospital Boston, Massachusetts 02115 USA
Publication Details: Cell, Vol.72(5), pp.767-778
Date published: 1993
Publisher: Elsevier Inc
Academic Unit: Biochemistry (NJMS)
Language: English
Resource Type: Journal article
Identifiers: 991031665649804646