Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2

Joon H Lee; Sang Ki Choi; Antonina Roll-Mecak; Stephen K Burley; Thomas E Dever

doi:10.1073/pnas.96.8.4342

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Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2

Journal article

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Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2

Joon H Lee, Sang Ki Choi, Antonina Roll-Mecak, Stephen K Burley and Thomas E Dever

Proceedings of the National Academy of Sciences - PNAS, Vol.96(8), pp.4342-4347

04/13/1999

DOI: https://doi.org/10.1073/pnas.96.8.4342

PMCID: PMC16334

PMID: 10200264

Abstract

Biological Sciences

eukaryotic translation initiation factor

FUN12

initiator tRNA

Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes by initiation factor (IF) IF2 and in eukaryotes by eIF2. The discovery of both IF2 and eIF2 homologs in yeast and archaea suggested that these microbes possess an evolutionarily intermediate protein synthesis apparatus. We describe the identification of a human IF2 homolog, and we demonstrate by using in vivo and in vitro assays that human IF2 functions as a translation factor. In addition, we show that archaea IF2 can substitute for its yeast homolog both in vivo and in vitro . We propose a universally conserved function for IF2 in facilitating the proper binding of initiator methionyl-tRNA to the ribosomal P site.

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Title: Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2
Creators: Joon H Lee - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716; and
Sang Ki Choi - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716; and
Antonina Roll-Mecak - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716; and
Stephen K Burley - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716; and
Thomas E Dever - Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716; and
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.96(8), pp.4342-4347
Date published: 04/13/1999
Publisher: The National Academy of Sciences
Academic Unit: Chemistry and Chemical Biology (SAS); Institute for Quantitative Biomedicine
Language: English
Resource Type: Journal article
Identifiers: 991031664324404646